Catalytic competence, a new criterion for affinity labeling. Demonstration of the reversible enzymatic interconversion of estrone and estradiol-17 beta covalently bound to human placental estradiol-17 beta dehydrogenase.
نویسندگان
چکیده
منابع مشابه
Catalytic Competence, a New Criterion for Affinity Labeling DEMONSTRATION OF THE REVERSIBLE ENZYMATIC INTERCONVERSION OF ESTRONE AND ESTRADIOL-17P COVALENTLY BOUND TO HUMAN PLACENTAL ESTRADIOL-170 DEHYDROGENASE*
Human placental estradiol-17P dehydrogenase is rapidly inactivated upon treatment with 3-bromoacetoxyestrone. Pseudo-first order kinetic data are obtained and inactivation is accompanied by incorporation of 1 mol of 3-acetoxyestrone/mol of subunit (M, = 34,000). Treatment of the inactivated enzyme with (4S)[4-2H]DPNH results in the formation of covalently bound [17cu-2H]estradiol-17/3, which ca...
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ACKNOWLEDGEMENTS Foremost, I would like to sincerely express my gratitude to my mentor Dr. James Simpkins for his continued support, encouragement, sound advice and for his strategic ideas that contributed to the betterment of my thesis work and helped me meet my timelines. This work would not have been possible without him. Also, I would like to sincerely appreciate Dr. Simpkins for his kind a...
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متن کاملSynthesis of 2-bromoacetamidoestrone methyl ether and study of the steroid-binding site of human placental estradiol 17 beta-dehydrogenase.
To characterize further the active site of human placental estradiol 17 beta-dehydrogenase (EC 1.1.1.62), we have synthesized 2-bromoacetamidoestrone methyl ether. The affinity-labeling steroid is a substrate for the homogeneous enzyme. It inactivates the enzyme in a time-dependent, irreversible manner which follows pseudo-first order kinetics. Further, inactivation conducted with varying stero...
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OBJECTIVES In earlier studies, we demonstrate that 17-beta -estradiol and an estrogen cell surface receptor can be found on various human cells, i.e., vascular endothelial, monocytes, and granulocytes, where they are coupled to nitric oxide release. We further demonstrated this phenomenon in the marine mussel Mytilus edulis ganglionic tissues. In the present report we sought to determine if est...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41202-7